Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal cells via a simple extraction method using animal cells cultured in 96-well (or other size well) plates. Direct RNA template purification from animal cells for use in real-time PCR takes approximately 10 minutes.
Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal cells via a simple extraction method using animal cells cultured in 96-well (or other size well) plates. Direct RNA template purification from animal cells for use in real-time PCR takes approximately 10 minutes.
When used with a one-step real-time RT-PCR kit, theCellAmp Direct RNA Prep Kit (Real Time) & Protein Analysis allows gene expression analysis to be completed in approximately two hours. When combined with a reverse transcription reagent such as the PrimeScript RT Reagent Kit (Perfect Real Time), cDNA templates for real-time PCR can be synthesized in approximately 30minutes, enabling efficient high-throughput analyses. Direct RNA template purification can be accomplished using small batches of animal cells, a useful feature for gene expression profiling of precious or rare samples. The kit is also useful for studies of genes that are expressed at very low levels. Genomic DNA can be easily and efficiently removed using this kit. Finally, RNA template samples prepared withCellAmp Direct RNA Prep Kit (Real Time) & Protein Analysis are compatible with intercalating green dye and 5" nuclease probe-based assays for real-time PCR (qPCR), and thus can serve a wide variety of experimental applications.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。