AMV Reverse Transcriptase XL synthesizes complementary full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates. The enzyme requires a primer to initiate synthesis and Mg2+ or Mn2+ for activity. AMV Reverse Transcriptase XL is positive for 5→3" ribonuclease H activity and can be used for a number of applications, including reverse transcription. AMV Reverse Transcriptase XL is highly purified, free of extraneous nucleases and is qualified for use in full-length cDNA synthesis and reverse transcription using RNA templates that are up to 10 kb in length. It can be used over a temperature range of 37–55°C, but maximal yield of full-length cDNA products isachieved between 41–44°C.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。