The NucleoSpin 96 PCR Clean-Up kits combine an improved buffer chemistry with the design of 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid cleanup of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.
The NucleoSpin 96 PCR Clean-Up kits combine an improved buffer chemistry with the design of 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid cleanup of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.
NucleoSpin PCR Clean-Up Binding Plates can be used under vacuum or by centrifugation. The NucleoSpin 96 PCR Clean-Up kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the silica membrane. PCR products bind to the silica membrane under optimized conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5 mM Tris/HCl pH 8.5) or water (pH 8.0–8.5) and are ready to use directly in subsequent applications.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。