The Western BLoT HRP substrate series are chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting. All of the substrates contain two components: a peroxide reagent and a luminol/enhancer solution. To use, equal volumes of the two solutions are mixed, the blot is incubated with the solution, and chemiluminescence is detected using film or digital imaging equipment. We offer four types of HRP substrates for Western blot detection.
The Western BLoT HRP Substrate series are chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting. All of the substrates contain two components, a peroxide reagent and a luminol/enhancer solution. To use, equal volumes of the two solutions are mixed, the blot is incubated with the solution, and chemiluminescence is detected using film or digital imaging equipment. We offer four types of HRP substrates for Western blot detection. Please refer to the table in the "More Information" section below to compare the substrates" specifications.
- The Western BLoT Chemiluminescence HRP Substrate (Cat. #T7101A) provides sensitivity suitable for detection using X-ray film. Although it is the most basic substrate in Western BLoT HRP substrate series, it produces a strong signal and provides high sensitivity with short exposure times.
- The Western BLoT Quant HRP Substrate (Cat. #T7102A) is intended for Western blot detection using a CCD camera. The signal from Western BLoT Quant is linear with respect to protein amount over a broad range of concentrations. In addition, this substrate produces a strong, long-lived signal, allowing long exposure times to enable the detection of low-abundance proteins. Western BLoT Quant substrate can also be used for X-ray film detection.
- The Western BLoT Hyper HRP Substrate (Cat. #T7103A) is more sensitive than Western BLoT Quant and is suitable for quantitative detection of low-abundance proteins. Western BLoT Hyper is also appropriate in situations when the amount of primary antibody is limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. Western BLoT Hyper substrate can also be used for X-ray film detection.
- The Western BLoT Ultra Sensitive HRP Substrate (Cat. #T7104A) is the most sensitive substrate in the series and is suitable for quantitative detection of very low-abundance proteins. It is possible to detect protein levels in the femtogram range using this product.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
暂无问答
本网站将在规定时间内给予删除等相关处理。
