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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/Human TCR profiling and differential expression on the ICELL8 cx system/Each/640192
产品编号:640192
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗人>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/Human TCR profiling and differential expression on the ICELL8 cx system/Each/640192
商品介绍

Single-cell TCR profiling on the ICELL8 cx system

Our high-throughput TCR profiling solution leverages SMART technology (Switching Mechanism At 5" End of RNA Template) and employs a 5" RACE-like approach to capture complete V(D)J variable regions of TCR transcripts from hundreds of single cells isolated on the ICELL8 cx system. As the name suggests, the workflow allows generation of libraries that provide information on both alpha- and beta-chain diversity.

Our high-throughput TCR profiling solution leverages SMART technology (Switching Mechanism at 5" End of RNA Template) and employs a 5" RACE-like approach to capture complete V(D)J variable regions of TCR transcripts from hundreds of single cells isolated on the ICELL8 cx system. As the name suggests, the workflow allows generation of libraries that provide information on both alpha- and beta-chain diversity.

The workflow incorporates Illumina-specific adapter sequences during cDNA amplification for the generation of indexed libraries that are ready for sequencing on Illumina platforms. The application also enables you to perform differential expression analysis by 5" end capture in parallel.

Single T cells isolated with the ICELL8 cx system, as well as positive and negative control samples, are dispensed into a 5,184-nanowell TCR chip that contains preprinted barcodes. Each TCR chip contains 1,728 unique barcodes, and each barcode is printed three times on the chip. Following identification of single-cell-bearing wells using ICELL8 cx CellSelect Software, SMART RT-PCR reagents are dispensed to the wells of samples chosen for further processing. Barcoded cDNAs from the selected wells are PCR-amplified in-chip. The full-length barcoded amplicons are then pooled off-chip, and the purified cDNA is used as template in the first TCR-specific PCR. The product from the first TCR-specific PCR is used as a template in a second TCR-specific PCR to incorporate Illumina indexed primers.

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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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