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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
产品编号:640143
市  场 价:¥590000.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$29500.00
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
商品介绍

NGS 3' DE workflow

By leveraging Takara Bio"s expertise in single-cell RNA-seq library construction and the cell isolation and selection capabilities of the ICELL8 platform, the ICELL8 3" DE and ICELL8 3" DE for UMI workflows enable high-throughput preparation of Illumina libraries for differential expression analysis of single cells from up to eight different samples in parallel.

By leveraging Takara Bio"s expertise in single-cell RNA-seq library construction and the cell isolation and selection capabilities of ICELL8 systems, the ICELL8 3" DE and ICELL8 3" DE for UMI workflows enable high-throughput preparation of Illumina libraries for differential expression analysis of single cells from up to eight different samples in parallel.

The ICELL8 Chip and Reagent 3" DE Kit (Cat. # 640164, 640165, 640166) is compatible with the original ICELL8 Single-Cell System. Included in the kit are an ICELL8 3" DE Chip (Cat. # 640143), a 5,184-nanowell SmartChip that contains preprinted oligo(dT) primers, and reagents for cDNA synthesis and Illumina library prep.

Following staining and dilution, sample cells are dispensed into chip nanowells using the ICELL8 MultiSample NanoDispenser (MSND). Wells containing single, viable cells are selectedusing the ICELL8 Imaging System and the CellSelect Software provided with the ICELL8 system. Following the selection of cells for processing and analysis, RT-PCR reagents are dispensed into the corresponding wells, and cDNA synthesis and amplification are performed in-chip in a single run. Following RT-PCR, cDNA products are pooled and purified, and sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3" ends, followed by a limited-cycle PCR amplification step that incorporates Illumina index sequences.

Sequencing data from the resulting libraries can be traced back to individual cells using the nanowell-specific barcode sequences and can be used to perform differential gene expression analysis by mRNA 3" end-counting. By yielding high-quality, 3"-focused sequencing data from hundreds of cells in parallel, these kits provide an efficient, cost-effective solution for high-throughput differential expression analysis of single cells.

The ICELL8 3" DE for UMI Reagent Kit is used with the ICELL8 3" DE Chip as part of the ICELL8 3" DE with UMI workflow, which enables Illumina sequencing and differential gene expression analysis-via transcript 3" end counting-of single cells isolated on the ICELL8 system.

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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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