The SMARTer Stranded RNA-Seq Kit provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA, and yields robust data from as little as100 pg to 100 ng of input RNA. The kit"s core SMART technology provides complete transcriptome coverage for both coding and noncoding RNA, and the incorporation of Illumina indexes and adapters is included in the workflow, eliminating the need for a downstream library preparation kit. For high-throughput applications, we also offer the SMARTer Stranded RNA-Seq Kit HT, which includes primer sets that allow for multiplexing of up to 96 sequencing libraries on a single flow-cell lane.

For rRNA removal from 10–100 ng of input total RNA, we recommend theRiboGone - Mammaliankit.For integrated rRNA depletion and library preparation for mammalian samplesfrom 250 pg–10 ng of total RNA, we recommend our SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian. For higher inputs (100 ng–1 µg of mammalian total RNA) we recommend our SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian.