These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for usingG418.
These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418. Transfected cells can also be used in assays that require fluorescent labeling of whole cells.
These vectors do not contain a multiple cloning site; other fluorescent protein vectors are available for cloning applications. Click here for green fluorescent protein options.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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