RHB-A is a fully defined, serum-free cell culture medium optimized for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This medium ensures the long-term stability of NS cell differentiation capacity (Sun et al. 2008) and can be used to support differentiation of human and mouse NS cells into functional neurons (Sun et al. 2008; Conti et al. 2005; Hansen et al. 2010) and of mouse ES cells into neural precursors (Ying et al. 2003; Diogo, Henrique, and Cabral 2008; Abranches et al. 2009). Critically, RHB-A medium is more efficient at neural differentiation than conventional N2- and B27-based methods. RHB-A medium can be used to support organotypic slice culture of central nervous system tissues such as brain and spinal cord, and supports the culture of glioblastoma stem cells used in cancer research.

NOTE: RHB-A medium requires supplementation with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor (FGF-2) for most applications (not supplied). RHB‐Basal medium does not contain any growth factors or neuronal supplements. Therefore, this medium can be tailored to the specific requirements of your cell type by the addition of supplements.