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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/PrimeSTAR HS DNA Polymerase/1,000 Units/R010B
产品编号:R010B
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/PrimeSTAR HS DNA Polymerase/1,000 Units/R010B
商品介绍

High-fidelity PCR

PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3" → 5" exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HSis available in flexible and convenient formats:

  • Individual components—PrimeSTAR HSDNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
  • 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.

PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3" → 5" exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HSis available in flexible and convenient formats:

  • Individual Components—PrimeSTAR HSDNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
  • 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.

The fidelity of PrimeSTAR HS DNA Polymerase was calculated by direct sequencing analysis to provide a more realistic estimate of the rate of incorporated errors compared with other commonly used indirect phenotypic analyses such as lacI. Calculation methods such as lacI, which are often used by other enzyme suppliers, are prone to error due to inherent lethal and silent mutations.

PrimeSTAR HS DNA Polymerase also offers excellent amplification efficiency and a fast reaction time due to its extremely high priming efficiency. Highly specific amplifications can be achieved using short annealing times of just 5–15 seconds.

For the most challenging PCR applications, enhanced versions of PrimeSTAR HS are available with the ability to amplify GC-rich sequences easily, even higher fidelity, and for long-range PCR:

  • PrimeSTAR HS with GC buffer provides reliable, high-fidelity amplification for the most difficult GC-rich content (75% or greater).
  • PrimeSTAR Max products provide the highest fidelity and fastest extension time of any Takara Bio product.
  • PrimeSTAR GXL products provide high fidelity plus the ability to amplify long and GC- or AT-rich targets.
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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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