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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/Luciferase reporters—Ready-To-Glow secreted luciferase assay/5,000 Rxns/631740
产品编号:631740
市  场 价:¥5780.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>抗体套装>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$289.00
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/Luciferase reporters—Ready-To-Glow secreted luciferase assay/5,000 Rxns/631740
商品介绍

The Ready-To-Glow Secreted Luciferase Reporter Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based assay.

Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using western blot analysis.

The Ready-To-Glow Secreted Luciferase Reporter Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system.

Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using western blot analysis.

Benefits of using a live-cell assay

Ready-To-Glow Secreted Luciferase Reporter AssayFirefly and Renilla-Based Reporter Assays
One-step protocol, without cell lysisYes. Reporter molecule is naturally secreted into the media and can be assayed without lysing cells.No.
2–4 fold higher signal than Renilla or firefly assaysYes.No.
Multiple data points per wellYes. Single well can be used for multiple time points, as there is no cell lysis.No.
Multiple compounds/trigger testing on same cells (single transfection)Yes.No.
Removal of background signalYes. Background signal can be reset to zero by removing and replacing the mediaNo.

Available format

The Ready-To-Glow Secreted Luciferase Reporter Assay includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.

Dual secreted reporter assay—luciferase & SEAP

The Ready-To-Glow Dual Secreted Reporter Assay has two live-cell reporters that do not require cell lysis. In addition to thesecreted luciferase assay,it includes a secretedversion ofalkaline phosphatase.

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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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