Apoptosis is associated with the fragmentation of chromosomal DNA into approximately 180-bp nucleosome fragments. The ApopLadder Ex kit permits the selective extraction of small, fragmented DNA from apoptotic cells while minimizing chromatin contamination. This kit also supports highly sensitive detection and quantitation of fragmented DNA. Detection of fragmented DNA following extraction can be performed via a DNA fragmentation assay involving gel electrophoresis, with gel-band quantification enabled by intercalating green dye fluorescent staining. The ApopLadder Ex kit contains the necessary reagents for cell lysis, enzymatic degradation of protein and RNA, precipitation of DNA fragments, and a 6X loading dye for gel electrophoresis.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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