PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript Reverse Transcriptase has exceptionally strong strand-displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile, and well-suited for applications requiring full-length cDNA, such as preparation of cDNA libraries and other techniques involving first-strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR).
PrimeScript RT can be used to perform reverse transcription from any RNA template, including GC-rich RNAs and those with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H-minus. Because of the excellent extension capability of PrimeScript RT, preparation of cDNAcan be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase was referenced in a variety of scientific applications such as gene expression profiling, gene discovery, transcriptome analysis, miRNA expression and regulation analysis, molecular evolution studies, virology, and microbiology.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。