The Apollo Library Prep System combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. You can also perform your TruSeq® stranded mRNA library prep, or generate VeriSeq™ PGS libraries, on the Apollo system to maximize your time and minimize sample loss. Preprogrammed scripts, including those validated for running the SMART-Seq v4 reagent kit, a ThruPLEX kit, Illumina TruSeq Stranded mRNA Library Prep kit, or VeriSeqPGS on the system, contribute to significantly reduced hands-on time compared to manual library prep methods. Simply set up, hit [RUN], and walk away to perform other tasks.

SMART-Seq v4 Reagent Kit for the Apollo System

This kit is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA using a high-throughput workflow that enables processing of up to 96 samples in a single working day. With this kit, we have reformatted our best-in-class method for ultra-low-input RNA-seq library prep for automation on the Apollo platform, providing a walkway solution that performs comparably to manual workflows. cDNA produced with this kit can be processed into NGS libraries suitable for analysis on Illumina or Ion Torrent platforms. The kit incorporates the same technologies and chemistries associated with our other SMART-Seq v4 products, including oligo(dT)-priming, SMART template-switching technology, and locked nucleic acids (LNA), ensuring the production of cDNA libraries that provide full-length sequence information, accurate representation of GC-rich transcripts, and industry-leading sensitivity.

ThruPLEX DNA Library Prep on the Apollo System

We adapted our trusted ThruPLEX chemistry for automation on the Apollo system, enabling you to start with 50 pg–30 ng of fragmented double-stranded DNA or 1–30 ng of cfDNA and efficiently process up to 48 samples per run, without wasting reagents. This versatile kit, combined with the convenience and consistency of the Apollo system, provides added sensitivity to better understand genetic variations within fragmented DNA samples from biofluids such as cell-free DNA, genomic DNA, DNA from FFPE materials, cDNA, or ChIP DNA. Once purified and quantified, the resulting library is ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System

Never perform Illumina TruSeq stranded mRNA library prep by hand again. Our automated protocol (download protocol) adapts the TruSeq Stranded mRNA library prep protocol for the Apollo system, allowing you to start with 0.1–1 µg of total RNA or 10–100 ng of previously isolated mRNA (from species with polyA tails) and process up to 48 samples per run, in multiples of six (Illumina Cat. # 20020594 for low-throughput or Cat. # 20020595 for high-throughput workflows). Once purified and quantified, the resulting library is ready for sequencing on Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated VeriSeq Library Prep for PGS on the Apollo System

Screen all 24 chromosomes for aneuploidy with the Illumina VeriSeq PGS Kit (Illumina Cat. # RH-101-1001) using our automated protocol (download protocol). This workflow leverages the Apollo system"s precision automation to allow the generation of up to 96 VeriSeq libraries in 13 hours, increasing reliability and efficiency by decreasing variability and human error. Following library generation and normalization, the resulting libraries are ready for Illumina NGS using standard Illumina sequencing reagents and protocols.