The NucleoSpin 8 Plasmid and NucleoSpin 8 Plasmid Core Kits are designed to purify up to 20 µg of high-copy plasmids from 1–5 ml of E. coli overnight cultures (preferably grown in LB medium). The NucleoSpin Plasmid Binding Strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.
Bacteria are harvested in an initial centrifugation step. After resuspension of the pelleted bacteria and alkaline cell lysis, the crude bacterial lysates are cleared by centrifugation or loaded onto NucleoSpin Plasmid Filter Strips arranged on the NucleoVac 96 vacuum manifold.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。