The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The protocol provided in the NucleoMag cfDNA user manual is intended for processing 2-ml inputs of blood plasma, but kit reagents are provided in sufficient quantities for processing input volumes ranging from 1–10 ml. In comparison with a competitor kit developed for cfDNA purification from plasma, NucleoMag cfDNA was shown to provide higher, more consistent yields from challenging samples containing low amounts of cfDNA.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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