The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit.
The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit. Carrier RNA improves binding and recovery of dilute viral nucleic acids. A combination of the supplied lysis buffer and ethanol create appropriate conditions for nucleic acids to bind to the NucleoSpin Virus Columns. After three washing steps to remove salts, metabolites, and soluble macromolecular cellular components, the nucleic acids are eluted in water and are ready for use in downstream reactions.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。