Our pathway profiling systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.
Our pathway profiling systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.
Profile multiple signal transduction pathways
Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor so that multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor vectors, indicating that a particular signaling pathway or pathways has been activated.
Two reporters available—luciferase & SEAP
- Luciferase induction can be measured from cell lysates using a standard assay.
- SEAP can be measured simply by collecting samples of the culture medium without cell lysis. Takara Bio offers the Great EscAPe SEAP Chemiluminescence Kit 2.0 to measure SEAP activity.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
暂无问答
本网站将在规定时间内给予删除等相关处理。
