In-Fusion Snap Assembly products enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing) is required. The efficiency of In-Fusion Snap Assembly is over 95%, providing confidence in your clones and enabling you to scale up for high-throughput workflows.
In-Fusion Snap Assembly products enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing) is required. The efficiency of In-Fusion Snap Assembly is over 95%, providing confidence in your clones and enabling you to scale up for high-throughput workflows.
The In-Fusion Snap Assembly Master Mix fuses PCR-generated sequences and linearized vectors efficiently and precisely—without the use of ligase—utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequences using our primer design tool. The In-Fusion master mix removes nucleotides from the 3" end of the linear DNA strands. This allows complementary base pairs between two pieces of DNA to anneal, leading to fragment joining. This method can be used to clone single or multiple fragments into a single vector without subcloning. Additional applications include mutagenesis, gene synthesis, gene design, domain swapping, and domain modification. The ability to clone directly into any destination vector at any locus (without the use of restriction enzymes) eliminates the need for any further subcloning or manipulation, simplifying the cloning workflow and further enabling a transition to high-throughput applications.
All In-Fusion Snap Assembly products contain the In-Fusion Snap Assembly Master Mix and reagents for control experiments. The master mix is provided in a liquid format as a 5X enzyme premix in 20-μl to 100-μl aliquots, depending on kit size.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。