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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/Single-cell full-length transcriptome analysis on the ICELL8 system powered by SMART-Seq technology/1 Chip/640183
产品编号:640183
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 其它>其它试剂>芯片>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/Single-cell full-length transcriptome analysis on the ICELL8 system powered by SMART-Seq technology/1 Chip/640183
商品介绍

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Full-length sequence information allows many types of analysis—such as differential gene expression, gene fusion, and alternative splicing—with high sensitivity. Our high-throughput solution leverages SMART-Seq chemistry for sensitive, full-length transcriptome profiling of 1,000–2,000 cells isolated on the ICELL8 Single-Cell System.

Please refer to the table in the "Components" section below for ordering information.

Full-length sequence information allows many types of analysis—such as differential gene expression, gene fusion, and alternative splicing—with high sensitivity. Our high-throughput solution leverages SMART-Seq chemistry for sensitive, full-length transcriptome profiling of 1,000–2,000 cells isolated on the ICELL8 Single-Cell System. The workflow incorporates Illumina-specific adapter sequences during cDNA amplification on an ICELL8 chip for the generation of indexed libraries that are ready for sequencing on Illumina platforms.

Please refer to the table in the "Components" section below for ordering information.

Single cells isolated with the ICELL8 Single-Cell System (Cat. # 640000) are dispensed into a 5,184-nanowell, blank ICELL8 250v Chip (Cat. # 640183). Following identification of single-cell-bearing wells using CellSelect Software, SMART RT-PCR reagents are then dispensed to the wells of samples chosen for further processing. The chip is then transferred to the ICELL8 Chip Cycler, which is programmed to perform one-step RT-PCR for first-strand synthesis and amplification of cDNA in a single run. The full-length cDNA is tagmented by TDE1 (Illumina Tagment DNA Enzyme), and then the tagmented cDNA is amplified using forward and reverse indexing primers (Cat. # 640205 and/or 640218). The resulting library is extracted, purified, and amplified to yield the final sequencing-ready library.

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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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