Strand-specific Illumina sequencing libraries from low RNA inputs of any quality

The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.

This low-input kit contains the Illumina Indexing Primer Set, and the entire protocol—from rRNA depletion through the creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.

Alternate kits for other input amounts:

• For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
• For 100 ng–1 µg of total RNA, we recommend the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian