In-Fusion HD EcoDry Cloning Plus products provide our In-Fusion HD cloning enzyme mix in a lyophilized, ready-to-use format that can be stored at room temperature. The cloning reagent is prealiquoted and dried down in tubes that are covered with an aluminum seal. This convenient cloning enzyme format is stable at room temperature, making it ideal for high-throughput cloning with robotic liquid handlers.
In-Fusion HD EcoDry Cloning Plus products provide our In-Fusion HD cloning enzyme mix in a lyophilized, ready-to-use format that can be stored at room temperature. The cloning reagent is pre-aliquoted and dried down in tubes that are covered with an aluminum seal. This convenient cloning enzyme format is stable at room temperature, making it ideal for high-throughput cloning with robotic liquid handlers. Whether at the bench or with automation platforms, use of pre-aliquoted reagents means minimal handling, eliminating chances of contamination, pipetting errors, or missing reagents. Just like the liquid format, In-Fusion HD EcoDry Cloning Plus allows you to clone any PCR fragment into any linearized vector in a single step without restriction digestion of the PCR fragment, ligation, or blunt-end polishing.
The ability to clone directly into any vector at any restriction site eliminates the need for any further subcloning or manipulation, greatly simplifying the cloning workflow and further enabling a transition to high-throughput applications. The In-Fusion HD enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequences using our primer design tool.
In-Fusion Cloning technology is based on the removal of nucleotides from the 3" end of the linear DNA strands. This allows complementary base pairs between two fragments of DNA base pairs to anneal, leading to fragment joining. This method can be used to clone single or multiple fragments into a single vector without subcloning. Additional applications include directional cDNA cloning, mutagenesis, gene synthesis, high-throughput cloning, as well as for the addition of adaptors, linkers, and protein tags.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。