Traditional methods of plant cell wall disruption that rely on tissue pulverization in liquid nitrogen using a mortar and pestle are not needed whentaking advantage of the Plant DNA Isolation Reagent. Thisplant DNA extraction kitmakes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition reduce heat treatment timeto only 15 minutes. In its entirety, theplant DNA extraction process can be completed within 30 minutes.Purified plant genomic DNA can be used directly for downstream applications such as PCR or restriction endonuclease digestion.
Traditional methods of plant cell wall disruption that rely on tissue pulverization in liquid nitrogen using a mortar and pestle are not needed whentaking advantage of the Plant DNA Isolation Reagent. Thisplant DNA extraction kitmakes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition reduce heat treatment timeto only 15 minutes. In its entirety, theplant DNA extraction process can be completed within 30 minutes.Purified plant genomic DNA can be used directly for downstream applications such as PCR or restriction endonuclease digestion.
Our Plant DNA Isolation Reagent is a ready-to-use plant DNA extractionkit that includes benzyl chloride forextraction ofplant genomic DNA. Benzyl chloride causes disintegration of plant cell walls by benzylation of the cellulose hydroxyl groups and other components of the plant cell wall. This kit takes advantage of benzyl chloride and uses it in a procedure that involves freezing the plant tissue, then thawing and grindingit with a pipette tip in a microcentrifuge tube. The freeze-thawing and grinding effectively degrade the plantcell wall.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。