Terra qPCR Direct TB Green Premix is a 2X master mix that lets you perform real-time PCR (qPCR) directly on crude extracts or dirty samples (such as mouse tail and ear biopsies, or leaf cuttings). The premix contains everything you need for qPCR, including Terra PCR Direct Polymerase—a robust, non-Taq polymerase developed for optimal amplification from whole cells, crude cell lysates, tissue extracts, or purified DNA. The enzyme is perfect for amplifying DNA targets up to 2 kb, even when the GC content is greater than 70%. The premix also contains TB Green dye, as well as a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing an automatic hot start. In addition, Terra qPCR Direct TB Green Premix comes packaged with two ROX formulations—ROX Reference Dye LSR and ROX Reference Dye LMP—allowing you to normalize the fluorescence signal between reactions on instruments that are equipped with this option.
Terra qPCR Direct TB Green Premix is a 2X master mix that lets you perform real-time PCR (qPCR) directly on crude extracts or dirty samples (such as mouse tail and ear biopsies, or leaf cuttings). The premix contains everything you need for qPCR, including Terra PCR Direct Polymerase—a robust, non-Taq polymerase developed for optimal amplification from whole cells, crude cell lysates, tissue extracts, or purified DNA. The enzyme is perfect for amplifying DNA targets up to 2 kb, even when the GC content is greater than 70%. The premix also contains TB Green dye, as well as a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing an automatic hot start. In addition, Terra qPCR Direct TB Green Premix comes packaged with two ROX formulations—ROX Reference Dye LSR and ROX Reference Dye LMP—allowing you to normalize the fluorescence signal between reactions on instruments that are equipped with this option.
Ready-to-use qPCR master mix containing TB Green is also available for cDNA templates.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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