With real-time PCR (qPCR), target-gene expression levels are often normalized to the expression of a reference gene to obtain relativequantificationof expression. This normalization strategy corrects for differences in the amount of input RNA and variation in reaction efficiency. A housekeeping gene that is stably expressed in all the samples being tested is commonly used as the reference. However, in some experimental systems, it may be difficult to identify a gene with stable expression. In such cases, an external standard RNA can be added to the reaction to serve as a reference.
With real-time PCR (qPCR), target-gene expression levels are often normalized to the expression of a reference gene to obtain relativequantificationof expression. This normalization strategy corrects for differences in the amount of input RNA and variation in reaction efficiency. A housekeeping gene that is stably expressed in all the samples being tested is commonly used as the reference. However, in some experimental systems, it may be difficult to identify a gene with stable expression. In such cases, an external standard RNA can be added to the reaction to serve as a reference.
External Standard Kit (Lambda PolyA) for qPCR provides an external reference for qPCR normalization. The kit contains the reference RNA (λpolyA+ RNA-A) and primers for real-time PCR detection. A fixed amount of theλpolyA RNA can be directly added to RNA samples before cDNA synthesis with oligo-dT. Subsequently, intercalating green dye-based qPCR detection can be used to quantify the amount ofλpolyA RNA in each sample.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。