SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage SP6 promoter sequence 5"-TAATCCACTGTGATAT-3". The enzyme can incorporate labeled or unlabeled ribonucleoside triphosphates into an RNA transcript. Large quantities of RNA can be synthesized from a DNA sequence cloned downstreamof the SP6 promoter due to the high processivity of SP6 RNA Polymerase. The enzyme is supplied in a buffer consisting of 10 mM potassium phosphate (pH 7.9), 150 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.
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