The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest.
The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest. All IRES selection vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene and allows preferential selection of cells expressing high levels of your protein of interest. The pBApo series of vectors are mammalian expression vectors for constitutive expression of your gene of interest from either a CMV or EF1-alpha promoter. The antibiotic resistance marker, either puromycin or neomycin, is expressed separately from an SV40 promoter. pBApo-CMV lacks a selectable marker.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。