pGBKT7 is a yeast two-hybrid bait expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.
pGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. To facilitate in vitro transcription/translation, pGADT7 AD includes the T7 promoter between the GAL4 AD and the epitope tag.
pGBKT7 is a yeast two-hybrid bait expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.
pGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. To facilitate in vitro transcription/translation, pGADT7 AD includes the T7 promoter between the GAL4 AD and the epitope tag.
The pBridge Vector allows you to investigate tertiary protein complexes with any GAL4-based two-hybrid system. pBridge may be used instead of pGBKT7 in the Matchmaker Gold System. With its two distinct multiple cloning sites, pBridge allows expression of a DNA-BD fusion protein and another distinct protein that acts as the "bridge" between the bait and prey (Tirode et al 1997; Brachmann and Boeke 1997).
A three-hybrid system allows you to investigate more complex protein interactions than are detected by a standard two-hybrid analysis. The third protein in this system can participate in the interaction in several ways:
Expression of the additional protein is controlled by a conditional methionine promoter—it is expressed in the absence of methionine. This allows expression to be switched on or off by culturing in media with or without methionine. The effect of the third protein is indicated by expression of a reporter or nutritional marker. We offer yeast media lacking methionine (Cat. # 630430, 630429) that are ideal for use with this vector.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。