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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Clontech/Protein transfection reagents/100 Rxns/631324
产品编号:631324
市  场 价:¥5780.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$289.00
品      牌: Clontech
公      司:Clontech
公司分类:
Clontech/Protein transfection reagents/100 Rxns/631324
商品介绍

Protein transfection reagents

The Xfect Protein Transfection Reagent uses a cell-penetrating peptide to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.

The Xfect Protein Transfection Reagent uses a cell-penetrating peptide to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.

What is the Xfect Protein Transfection Reagent and how does it work?

Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. Just incubate your protein of interest with the Xfect reagent in the supplied buffer for 30 minutes and then apply the mixture to your cells. Two hours later you are ready to assay for protein activity.

Why transfect purified proteins directly?

Protein transfection is extremely rapid compared to traditional gene expression studies using transfected DNA (1–2 hours compared to 18–48 hours) because it bypasses cellular processes such as transcription and translation. It also facilitates studies involving transient effects of proteins, and avoids potentially harmful random DNA integration into the genome of the target cells. Xfect Protein Transfection Reagent makes it possible to deliver active proteins directly into cells for studies that involve transcriptional regulation, the cell cycle, apoptosis, oncogenesis, epigenetics, cell regeneration, and transdifferentiation.

What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?

The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Lipid-based reagents tend to demonstrate high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both, i.e., it retains low cytotoxicity and delivers more protein to a higher percentage of target cells (see Image Data tab in the product details section below). Moreover, Xfect Protein Transfection Reagent can transfect cells that are growing at a higher density than competing products, which is important since assays are often performed within a few hours post-transfection and the higher cell densities ensure sufficient material for downstream analysis.

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品牌介绍

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。

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