Our pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome-associated chaperone protein of 48 kDa which facilitates co-translational folding of nascent polypeptides, reducing protein misfolding and production of insoluble protein. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNA vector contains a cspA promoter plus additional downstream sequences including a 5" untranslated region (5" UTR), a translation enhancing element (TEE), a his-tag sequence and a multiple cloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin and Factor Xa are located between theMCS and TF/His fusion tags and function to facilitate tag removal from the expressed fusion protein. Most E. coli strains can serve as expression hosts.
GenBank Accession No: AB213654
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT-PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品,其强大的科研实力和完善的质量管理体系(ISO9001)为Clontech产品的良好品质提供了可靠保障。