With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied columns, which are suitable for gel extraction as well as PCR purification, bind DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded onto the column, contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3—providing rapid DNA purification.
With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied columns, which are suitable for gel extraction as well as PCR purification, bind DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded onto the column, contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in minimal elution volumes (as low as 15 µl for the mini kit, 200 µl for the midi kit, and 1 ml for the maxi kit) with recovery rates of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.
The NucleoSpin Gel and PCR Clean-Up kit was formerly known as the NucleoSpin Extract II kit.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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