The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells.
The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells. High-titer AAV2 particles can be prepared without a helper virus. HEK 293T cells are transfected with plasmids encoded in this kit, and AAVpro Extraction Solution is used for efficient AAV2 particle isolation.
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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